PIGA antibody (AA 179-228)

Catalog No. ABIN6744797

The Rabbit Polyclonal anti-PIGA antibody has been validated for WB. It is suitable to detect PIGA in samples from Human, Mouse, Cow, Pig, Rabbit, Dog, Horse, Bat and Monkey.

Quick Overview for PIGA antibody (AA 179-228) (ABIN6744797)

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Reactivity: Human, Mouse, Cow, Pig, Rabbit, Dog, Horse, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIGA Antibody

Binding Specificity: AA 179-228

Specificity: Human PIGA

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Pig (100%) Rat, Chicken, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa179-228 of human PIGA (P37287, NP_002632). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Platypus, Lizard (100%), Rat, Hamster, Turkey, Zebra finch, Chicken, Zebrafish (92%), Xenopus (90%), Guinea pig, Stickleback (85%), Panda (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PIGA

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Alternative Name: GPI3 / PIGA

Background: Name/Gene ID: PIGA

Synonyms: PIGA, GPI anchor biosynthesis, GPI3, PIG-A, GlcNAc-PI synthesis protein, MCAHS2

Gene ID: 5277

NCBI Accession: NP_002632

UniProt: P37287

Pathways: Inositol Metabolic Process