PIGA antibody (AA 410-490)

Catalog No. ABIN7230638

The Rabbit Polyclonal anti-PIGA antibody is suitable to detect PIGA in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for PIGA antibody (AA 410-490) (ABIN7230638)

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PIGA Antibody

Binding Specificity: AA 410-490

Purpose: Rabbit Anti-PIGA Polyclonal Antibody

Specificity: The antibody detects endogenous levels of PIGA protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human PIGA protein at AA range: 410-490

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PIGA

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Alternative Name: PIGA

Background: Phosphatidylinositol N-acetylglucosaminyltransferase subunit A, GlcNAc-PI synthesis protein, Phosphatidylinositol-glycan biosynthesis class A protein, PIG-APIGA (Phosphatidylinositol Glycan Anchor Biosynthesis Class A) is a Protein Coding gene. Diseases associated with PIGA include Multiple Congenital Anomalies-Hypotonia-Seizures Syndrome 2 and Paroxysmal Nocturnal Hemoglobinuria, Somatic. Among its related pathways are Glycosylphosphatidylinositol (GPI)-anchor biosynthesis and Metabolism. PIGA encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12.

Molecular Weight: 53kD

Gene ID: 5277

UniProt: P37287

Pathways: Inositol Metabolic Process