POLI antibody (AA 143-192)

Catalog No. ABIN6744961

The Rabbit Polyclonal anti-POLI antibody has been validated for WB. It is suitable to detect POLI in samples from Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat, Monkey and Pig.

Quick Overview for POLI antibody (AA 143-192) (ABIN6744961)

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLI antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLI Antibody

Binding Specificity: AA 143-192

Specificity: Human POLI

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig (100%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa143-192 of human POLI (Q9UNA4, NP_009126). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Horse, Pig, Guinea pig (100%), Elephant, Platypus (92%), Opossum, Salmon, Zebrafish (85%), Stickleback (84%), Drosophila (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLI

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Alternative Name: POLI

Background: Name/Gene ID: POLI

Synonyms: POLI, DNA polymerase iota, Eta2, Polymerase (DNA directed) iota, RAD30 homolog B, RAD3OB, RAD30B

Gene ID: 11201

NCBI Accession: NP_009126

UniProt: Q9UNA4

Pathways: DNA Damage Repair