LIG1 antibody (AA 611-660)

Catalog No. ABIN6745053

The Rabbit Polyclonal anti-LIG1 antibody is suitable to detect LIG1 in samples from Human, Rat, Mouse, Rabbit and Monkey. It has been validated for WB.

Quick Overview for LIG1 antibody (AA 611-660) (ABIN6745053)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human, Rat, Mouse, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LIG1 Antibody

Binding Specificity: AA 611-660

Specificity: Human LIG1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Dog, Bovine, Horse, Pig (92%) Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa611-660 of human LIG1 (P18858, NP_000225). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat (100%), Elephant, Dog, Bovine, Bat, Horse, Pig, Guinea pig, Platypus, Xenopus, Pufferfish (92%), Rabbit (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: LIG1 / DNA Ligase 1

Background: Name/Gene ID: LIG1

Synonyms: LIG1, DNA ligase I, DNA ligase 1, Ligase I, DNA, ATP-dependent

Gene ID: 3978

NCBI Accession: NP_000225

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA