APEX1 antibody (AA 215-264)

Catalog No. ABIN6745230

This anti-APEX1 antibody is a Rabbit Polyclonal antibody detecting APEX1 in WB. Suitable for Human, Mouse, Rat, Cow, Pig, Dog, Rabbit, Horse, Guinea Pig, Goat, Monkey, Bat, Zebrafish (Danio rerio) and Hamster.

Quick Overview for APEX1 antibody (AA 215-264) (ABIN6745230)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Rabbit, Horse, Guinea Pig, Goat, Monkey, Bat, Zebrafish (Danio rerio), Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APEX1 Antibody

Binding Specificity: AA 215-264

Specificity: Human APEX1 / APE1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Goat, Pig (100%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa215-264 of human APEX1 (P27695, NP_001632). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Tamarin, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus (100%), Salmon, Sablefish, Stickleback, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX1 / APE1

Background: Name/Gene ID: APEX1

Synonyms: APEX1, AP endonuclease class I, AP lyase, APE, APE-1, APEN, APE1, APEX nuclease, Redox factor-1, AP endonuclease 1, APEX, APX, Protein REF-1, REF-1, REF1

Gene ID: 328

NCBI Accession: NP_001632

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus