UCHL1 antibody (C-Term)

Catalog No. ABIN6745386

The Rabbit Polyclonal anti-UCHL1 antibody has been validated for WB. It is suitable to detect UCHL1 in samples from Human, Rat, Mouse, Cow, Pig, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Monkey, Horse and Bat.

Quick Overview for UCHL1 antibody (C-Term) (ABIN6745386)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human, Rat, Mouse, Cow, Pig, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Monkey, Horse, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UCHL1 Antibody

Binding Specificity: C-Term

Specificity: Human UCHL1 / PGP9.5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse, Pig, Guinea pig (100%) Dog, Xenopus (92%) Chicken, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human UCHL1 (P09936, NP_004172). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Dog, Lizard, Xenopus (92%), Opossum, Turkey, Zebra finch, Chicken, Stickleback, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: UCHL1 / PGP9.5

Background: Name/Gene ID: UCHL1
Subfamily: Cysteine C12
Family: Protease

Synonyms: UCHL1, Ubiquitin C-terminal hydrolase, PARK5, PGP9.5, PGP95, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Uch-L1

Gene ID: 7345

NCBI Accession: NP_004172

UniProt: P09936

Pathways: Feeding Behaviour