14-3-3 sigma/SFN antibody (AA 51-100)

Catalog No. ABIN6745577

The Rabbit Polyclonal anti-14-3-3 sigma/SFN antibody has been validated for WB. It is suitable to detect 14-3-3 sigma/SFN in samples from Human, Mouse, Rat, Dog, Guinea Pig, Monkey, Pig and Hamster.

Quick Overview for 14-3-3 sigma/SFN antibody (AA 51-100) (ABIN6745577)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Monkey, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-14-3-3 sigma/SFN Antibody

Binding Specificity: AA 51-100

Specificity: Human SFN / 14-3-3 Sigma

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Pig, Guinea pig (100%) Rabbit (92%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa51-100 of human SFN (P31947, NP_006133). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Pig, Guinea pig (100%), Sheep, Bovine, Rabbit, Horse (92%), Dog (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: SFN / Stratifin / 14-3-3 Sigma

Background: Name/Gene ID: SFN

Synonyms: SFN, 14-3-3 sigma, 14-3-3 protein sigma, HME1, Stratifin, YWHAS

Gene ID: 2810

NCBI Accession: NP_006133

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction