HGF antibody (AA 216-265)

Catalog No. ABIN604744

Product Details anti-HGF Antibody

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Binding Specificity: AA 216-265

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Cow, Horse, Guinea Pig, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human HGF

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig (100%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa216-265 of human HGF (P14210, NP_000592). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Cat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Bat (92%), Turkey, Zebra finch, Chicken (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HGF

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Alternative Name: HGF (HGF Products)

Background: Name/Gene ID: HGF
Subfamily: Serine S1
Family: Protease

Synonyms: HGF, DFNB39, Hepatocyte growth factor, Hepatopoeitin-A, Hepapoietin A, Hepatopoietin-A, Scatter factor), SF, F-TCF, HGFB, HPTA, Scatter factor

Gene ID: 3082

NCBI Accession: NP_000592

UniProt: P14210

Pathways: RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor