HGF antibody (AA 306-471)

Catalog No. ABIN7430570

The Rabbit Polyclonal anti-HGF antibody is suitable to detect HGF in samples from Rat. It has been validated for WB, IHC, IP and ICC.

Quick Overview for HGF antibody (AA 306-471) (ABIN7430570)

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-HGF Antibody

Binding Specificity: AA 306-471

Purpose: Polyclonal Antibody to Hepatocyte Growth Factor (HGF)

Specificity: The antibody is a rabbit polyclonal antibody raised against HGF. It has been selected for its ability to recognize HGF in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Hepatocyte Growth Factor (HGF) corresdonding to Cys306~Glu471 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HGF

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Alternative Name: Hepatocyte Growth Factor

Background: HGF, F-TCF, HGFB, HPTA, SF, Scatter Factor, Hepapoietin A, Fibroblast-Derived Tumor Cytotoxic Factor, Lung Fibroblast-Derived Mitogen

UniProt: P17945

Pathways: RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor