HGF antibody (AA 495-728)

Catalog No. ABIN7425623

The Mouse Monoclonal anti-HGF antibody is suitable to detect HGF in samples from Human. It has been validated for WB and IHC.

Quick Overview for HGF antibody (AA 495-728) (ABIN7425623)

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C1

Product Details anti-HGF Antibody

Binding Specificity: AA 495-728

Purpose: Monoclonal Antibody to Hepatocyte Growth Factor (HGF)

Specificity: The antibody is a mouse monoclonal antibody raised against HGF. It has been selected for its ability to recognize HGF in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Hepatocyte Growth Factor (HGF) corresdonding to Val495~Ser728 with N-terminal His Tag

Isotype: IgG2a kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HGF

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Alternative Name: Hepatocyte Growth Factor

Background: HGF, F-TCF, HGFB, HPTA, SF, Scatter Factor, Hepapoietin A, Fibroblast-Derived Tumor Cytotoxic Factor, Lung Fibroblast-Derived Mitogen

Pathways: RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor