ABCB1 antibody (AA 863-912)

Catalog No. ABIN6745748

This Rabbit Polyclonal antibody specifically detects ABCB1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Rabbit and Hamster.

Quick Overview for ABCB1 antibody (AA 863-912) (ABIN6745748)

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Reactivity: Human, Mouse, Rat, Rabbit, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ABCB1 Antibody

Binding Specificity: AA 863-912

Brand: IHC-plus™

Specificity: Human ABCB1 / P Glycoprotein

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit (100%) Sheep, Dog, Bovine, Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa863-912 of human ABCB1 (P08183, NP_000918). Percent identity by BLAST analysis: Human, Mouse, Rat, Hamster, Rabbit (100%), Gorilla, Gibbon, Monkey, Galago, Sheep, Panda, Dog, Cat, Bovine, Bat, Pig (92%), Marmoset, Horse (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ABCB1

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Alternative Name: ABCB1 / MDR1 / P Glycoprotein

Background: Name/Gene ID: ABCB1
Subfamily: ATP-binding cassette - ABCB/MDR
Family: Transporter

Synonyms: ABCB1, ABC20, Abcb1b, CLCS, Colchicin sensitivity, CD243, Doxorubicin resistance, IBD13, gp170, MDR1, Multidrug resistance protein 1, P glycoprotein, P-glycoprotein 1, P-GP, CD243 antigen, PGY1

Gene ID: 5243

NCBI Accession: NP_000918

UniProt: P08183