HNF1A antibody (AA 179-228)

Catalog No. ABIN6745749

The Rabbit Polyclonal anti-HNF1A antibody has been validated for WB, IHC and IHC (p). It is suitable to detect HNF1A in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig and Bat.

Quick Overview for HNF1A antibody (AA 179-228) (ABIN6745749)

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HNF1A Antibody

Binding Specificity: AA 179-228

Brand: IHC-plus™

Specificity: Human HNF1A / TCF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Pig (100%) Bovine (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa179-228 of human HNF1A (P20823, NP_000536). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Mouse, Rat, Elephant, Dog, Bat, Horse, Pig, Guinea pig (100%), Panda, Bovine, Opossum, Platypus (92%), Turkey, Zebra finch, Chicken (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HNF1A

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Alternative Name: HNF1A / HNF1

Background: Name/Gene ID: HNF1A
Family: Transcription factor

Synonyms: HNF1A, Albumin proximal factor, HNF1, Hepatic nuclear factor 1, HNF-1-alpha, MODY3, HNF-1A, IDDM20, LFB1, TCF1, Transcription factor 1, HNF1 homeobox A, TCF-1

Gene ID: 6927

NCBI Accession: NP_000536

Pathways: Hormone Transport, Carbohydrate Homeostasis