K-RAS antibody (N-Term)

Catalog No. ABIN604770

The Rabbit Polyclonal anti-K-RAS antibody (ABIN604770) specifically detects K-RAS in WB, IHC and IHC (p). The antibody is reactive with Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Cow, Drosophila melanogaster, Pig, Bat, Chicken, Xenopus laevis, Hamster and Monkey samples.

Quick Overview for K-RAS antibody (N-Term) (ABIN604770)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Cow, Drosophila melanogaster, Pig, Bat, Chicken, Xenopus laevis, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This K-RAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-K-RAS Antibody

Binding Specificity: N-Term

Specificity: Human KRAS

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Rabbit, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human KRAS (P01111, NP_203524). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus, Frog, Catfish, Salmon, Smelt, Medaka, Pufferfish, Drosophila (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: KRAS

Background: Name/Gene ID: KRAS
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: KRAS, C-K-RAS, C-Ki-ras, C-Kirsten-ras protein, GTPase KRas, K-ras p21 protein, K-RAS2B, K-RAS2, KRAS2, NS, NS3, K-RAS4A, KRAS1, RASK2, Transforming protein p21, Oncogene KRAS2, K-Ras 2, K-RAS2A, K-RAS4B, KI-RAS, PR310 c-K-ras oncogene

Gene ID: 3845

NCBI Accession: NP_203524

UniProt: P01116