CD25 antibody

Catalog No. ABIN6745801

This Mouse Monoclonal antibody specifically detects CD25 in FACS, IHC, IP and IHC (fro). It exhibits reactivity toward Cow and Sheep.

Quick Overview for CD25 antibody (ABIN6745801)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Cow, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD25 Antibody

Specificity: Recognizes the bovine Interleukin-2 receptor alpha chain, a 55kD glycoprotein expressed by activated T cells. Blocks the IL-2 driven proliferation of Con A-induced blast cells/ bovine lymphocytes. Species cross-reactivity: sheep.

Purification: Protein G purified

Immunogen: Bovine Interleukin 2 Receptor, alpha.

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IP

Usage: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Flow Cytometry: 1:50-1:100, 10 μL labels 10^6 cells in 100 μL.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: IL2RA / CD25

Background: Name/Gene ID: IL2RA
Family: Interleukin

Synonyms: IL2RA, CD25 antigen, CD25, IDDM10, IL-2 receptor subunit alpha, IL2R, Interleukin 2 receptor, alpha, IL-2-RA, IL2-RA, TCGFR, IL-2R subunit alpha, p55, TAC antigen

Gene ID: 3559

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation