PYCARD antibody (AA 121-170)

Catalog No. ABIN6746135

The Rabbit Polyclonal anti-PYCARD antibody has been validated for WB. It is suitable to detect PYCARD in samples from Human and Rat. There are 4+ publications available.

Quick Overview for PYCARD antibody (AA 121-170) (ABIN6746135)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PYCARD Antibody

Binding Specificity: AA 121-170

Specificity: Human PYCARD

Predicted Reactivity: Percent identity by BLAST analysis:

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa121-170 of human PYCARD (Q9ULZ3, NP_037390). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%), Marmoset (92%), Hamster, Panda, Bat, Rabbit, Horse, Pig (85%), Rat (78%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PYCARD antibody (ABIN6746135)

Nakashio, Fujita, Rokudai, Sato, Tsuruo: "Prevention of phosphatidylinositol 3'-kinase-Akt survival signaling pathway during topotecan-induced apoptosis." in: Cancer research, Vol. 60, Issue 18, pp. 5303-9, (2000) (PubMed).

Auphan, DiDonato, Rosette, Helmberg, Karin: "Immunosuppression by glucocorticoids: inhibition of NF-kappa B activity through induction of I kappa B synthesis." in: Science (New York, N.Y.), Vol. 270, Issue 5234, pp. 286-90, (1995) (PubMed).

Cordle, Donald, Read, Hawiger: "Lipopolysaccharide induces phosphorylation of MAD3 and activation of c-Rel and related NF-kappa B proteins in human monocytic THP-1 cells." in: The Journal of biological chemistry, Vol. 268, Issue 16, pp. 11803-10, (1993) (PubMed).

Haskill, Beg, Tompkins, Morris, Yurochko, Sampson-Johannes, Mondal, Ralph, Baldwin: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, (1991) (PubMed).

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: PYCARD / ASC

Background: Name/Gene ID: PYCARD
Family: Apoptosis

Synonyms: PYCARD, CARD5, HASC, ASC, TMS, TMS-1, PYD and CARD domain containing, TMS1

Gene ID: 29108

NCBI Accession: NP_037390

UniProt: Q9ULZ3

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome