G Substrate antibody (AA 35-84)

Catalog No. ABIN6746344

The Rabbit Polyclonal anti-G Substrate antibody has been validated for WB. It is suitable to detect G Substrate in samples from Human, Mouse, Pig and Rat. There are 3+ publications available.

Quick Overview for G Substrate antibody (AA 35-84) (ABIN6746344)

Target: G Substrate (Gsbs)

Reactivity: Human, Mouse, Pig, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G Substrate antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-G Substrate Antibody

Binding Specificity: AA 35-84

Specificity: Human C7ORF16

Predicted Reactivity: Percent identity by BLAST analysis:

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human PPP1R17 (O96001, NP_006649). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Elephant, Pig (100%), Marmoset, Dog, Bovine, Horse, Guinea pig (92%), Turkey, Chicken (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-G Substrate antibody (ABIN6746344)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Christmann, Kaina: "Nuclear translocation of mismatch repair proteins MSH2 and MSH6 as a response of cells to alkylating agents." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36256-62, (2000) (PubMed).

Palombo, Gallinari, Iaccarino, Lettieri, Hughes, DArrigo, Truong, Hsuan, Jiricny: "GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells." in: Science (New York, N.Y.), Vol. 268, Issue 5219, pp. 1912-4, (1995) (PubMed).

Target Details for G Substrate

Target: G Substrate (Gsbs)

Alternative Name: PPP1R17 / C7orf16

Background: Name/Gene ID: PPP1R17

Synonyms: PPP1R17, G-substrate, GSBS, C7orf16

Gene ID: 10842

NCBI Accession: NP_006649

UniProt: O96001