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IL-5 antibody (AA 21-70)

This Rabbit Polyclonal antibody specifically detects IL-5 in WB. It exhibits reactivity toward Human, Mouse, Rat and Guinea Pigand has been mentioned in 1 publication.
Catalog No. ABIN6746379

Quick Overview for IL-5 antibody (AA 21-70) (ABIN6746379)

Target

See all IL-5 (IL5) Antibodies
IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity

  • 73
  • 41
  • 20
  • 12
  • 7
  • 6
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Human, Mouse, Rat, Guinea Pig

Host

  • 62
  • 31
  • 30
  • 2
  • 2
Rabbit

Clonality

  • 62
  • 61
  • 3
Polyclonal

Conjugate

  • 95
  • 14
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This IL-5 antibody is un-conjugated

Application

  • 86
  • 47
  • 41
  • 37
  • 32
  • 19
  • 13
  • 13
  • 12
  • 11
  • 7
  • 6
  • 5
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 21
    • 15
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 21-70

    Specificity

    Mouse IL5 / IL-5

    Predicted Reactivity

    Percent identity by BLAST analysis: Human (100%) Guinea pig (85%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide located between aa21-70 of mouse Il5 (P04401, NP_034688). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse (100%), Baboon, Marmoset, Rabbit (92%), Guinea pig (85%), Rat (78%).

    Type of Immunogen: Synthetic peptide
  • Application Notes

    Approved: WB (0.2 - 1 μg/mL)

    Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

    Comment

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Beckhelling, Chang, Chevalier, Ford, Houliston: "Pre-M phase-promoting factor associates with annulate lamellae in Xenopus oocytes and egg extracts." in: Molecular biology of the cell, Vol. 14, Issue 3, pp. 1125-37, (2003) (PubMed).

  • Target

    IL-5 (IL5) (Interleukin 5 (IL5))

    Alternative Name

    IL5

    Background

    Name/Gene ID: IL5
    Family: Hormone

    Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF

    Gene ID

    3567

    NCBI Accession

    NP_034688

    UniProt

    P05113

    Pathways

    JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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