MAPK12 antibody (AA 289-338)

Catalog No. ABIN6746453

The Rabbit Polyclonal anti-MAPK12 antibody has been validated for WB. It is suitable to detect MAPK12 in samples from Human, Mouse, Rat, Rabbit and Cow. There are 2+ publications available.

Quick Overview for MAPK12 antibody (AA 289-338) (ABIN6746453)

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Reactivity: Human, Mouse, Rat, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAPK12 Antibody

Binding Specificity: AA 289-338

Specificity: Mouse MAPK12 / ERK5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit (100%) Dog, Horse, Pig, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa289-338 of mouse Mapk12 (O08911, NP_038899). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Elephant, Rabbit, Opossum (100%), Marmoset, Dog, Bat, Horse, Pig, Guinea pig (92%), Galago, Panda (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-MAPK12 antibody (ABIN6746453)

Fulda, Meyer, Debatin: "Inhibition of TRAIL-induced apoptosis by Bcl-2 overexpression." in: Oncogene, Vol. 21, Issue 15, pp. 2283-94, (2002) (PubMed).

Li, Chen, Sinogeeva, Gorospe, Wersto, Chrest, Barnes, Liu: "Arsenic trioxide promotes histone H3 phosphoacetylation at the chromatin of CASPASE-10 in acute promyelocytic leukemia cells." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49504-10, (2002) (PubMed).

Target Details for MAPK12

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Alternative Name: MAPK12 / ERK6 / SAPK3

Background: Name/Gene ID: MAPK12
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK12, ERK5, ERK6, ERK-6, MAP kinase 12, MAPK 12, MAP kinase p38 gamma, SAPK3, p38-gamma, p38GAMMA, SAPK-3, ERK3, PRKM12

Gene ID: 6300

NCBI Accession: NP_038899

UniProt: P53778

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C, BCR Signaling, S100 Proteins