Osteopontin antibody (N-Term)

Catalog No. ABIN6747021

This anti-Osteopontin antibody is a Rabbit Polyclonal antibody detecting Osteopontin in WB, IHC and IHC (p). Suitable for Human, Rabbit, Guinea Pig and Horse.

Quick Overview for Osteopontin antibody (N-Term) (ABIN6747021)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Human, Rabbit, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteopontin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Osteopontin Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human SPP1 / Osteopontin

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog (92%) Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human SPP1 (B2RDA1, NP_000573). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Elephant, Rabbit, Horse (100%), Gibbon, Marmoset, Panda, Dog (92%), Guinea pig (85%), Platypus (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: SPP1 / Osteopontin

Background: Name/Gene ID: SPP1

Synonyms: SPP1, BSPI, BNSP, Bone sialoprotein 1, ETA-1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin, Osteopontin, Bone sialoprotein I

Gene ID: 6696

NCBI Accession: NP_000573

UniProt: P10451

Pathways: Regulation of Cell Size