ACTN3 antibody (AA 180-229)

Catalog No. ABIN6747075

The Rabbit Polyclonal anti-ACTN3 antibody (ABIN6747075) specifically detects ACTN3 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse, Hamster, Monkey and Pig samples.

Quick Overview for ACTN3 antibody (AA 180-229) (ABIN6747075)

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACTN3 Antibody

Binding Specificity: AA 180-229

Specificity: Human ACTN3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse (100%) Zebrafish (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human ACTN3 (Q08043, NP_001095). Percent identity by BLAST analysis: Human, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Gibbon, Xenopus, Zebrafish (92%), Gorilla, Elephant, Turkey, Zebra finch, Chicken (85%), Galago, Bat, Lizard, Salmon, Stickleback, Drosophila (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ACTN3

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Alternative Name: ACTN3

Background: Name/Gene ID: ACTN3

Synonyms: ACTN3, Actinin, alpha 3, Alpha-actinin skeletal muscle, Alpha-actinin-3, F-actin cross-linking protein

Gene ID: 89

NCBI Accession: NP_001095

UniProt: Q08043

Pathways: Cell-Cell Junction Organization