CHFR antibody (AA 36-85)

Catalog No. ABIN6747082

This Rabbit Polyclonal antibody specifically detects CHFR in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Rabbit, Zebrafish (Danio rerio) and Monkey.

Quick Overview for CHFR antibody (AA 36-85) (ABIN6747082)

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHFR Antibody

Binding Specificity: AA 36-85

Brand: IHC-plus™

Specificity: Human CHFR

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Guinea pig (100%) Dog, Bovine, Horse, Pig, Chicken (92%) Xenopus, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human CHFR (Q96EP1, NP_060693). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Rabbit, Guinea pig (100%), Elephant, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus (92%), Xenopus, Salmon, Zebrafish (85%), Stickleback (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CHFR

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Alternative Name: CHFR

Background: Name/Gene ID: CHFR

Synonyms: CHFR, RING finger protein 196, RNF196, RNF116

Gene ID: 55743

NCBI Accession: NP_060693