TBK1 antibody (AA 539-588)

Catalog No. ABIN6747170

The Rabbit Polyclonal anti-TBK1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect TBK1 in samples from Human, Mouse, Rat, Cow, Rabbit, Dog, Monkey, Pig, Horse and Guinea Pig.

Quick Overview for TBK1 antibody (AA 539-588) (ABIN6747170)

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Monkey, Pig, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TBK1 Antibody

Binding Specificity: AA 539-588

Brand: IHC-plus™

Specificity: Human TBK1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig (100%) Chicken, Zebrafish (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa539-588 of human TBK1 (Q9UHD2, NP_037386). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Platypus (100%), Zebra finch, Chicken, Lizard, Stickleback, Zebrafish (92%), Bat, Turkey, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TBK1

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Alternative Name: NAK / TBK1

Background: Name/Gene ID: TBK1
Subfamily: IKAPPAB
Family: Protein Kinase

Synonyms: TBK1, T2K, NAK, NF-kappa-B-activating kinase, NF-kB-activating kinase, TANK-binding kinase 1

Gene ID: 29110

NCBI Accession: NP_037386

UniProt: Q9UHD2

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome