ALKBH3 antibody (AA 216-265)

Catalog No. ABIN6747175

The Rabbit Polyclonal anti-ALKBH3 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect ALKBH3 in samples from Human, Mouse, Rat, Horse, Rabbit, Bat and Monkey.

Quick Overview for ALKBH3 antibody (AA 216-265) (ABIN6747175)

Target: ALKBH3 (AlkB, Alkylation Repair Homolog 3 (ALKBH3))

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ALKBH3 Antibody

Binding Specificity: AA 216-265

Brand: IHC-plus™

Specificity: Human ALKBH3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Horse (100%) Dog, Guinea pig, Chicken, Xenopus (92%) Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa216-265 of human ALKBH3 (Q96Q83, NP_631917). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bat, Horse, Smelt (100%), Dog, Bovine, Guinea pig, Chicken, Platypus, Xenopus, Stickleback (92%), Rabbit, Turkey, Catfish (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ALKBH3

Target: ALKBH3 (AlkB, Alkylation Repair Homolog 3 (ALKBH3))

Alternative Name: PCA1 / ALKBH3

Background: Name/Gene ID: ALKBH3

Synonyms: ALKBH3, ABH3, DEPC1, DEPC-1, Prostate cancer antigen 1, PCA1, Prostate cancer antigen-1

Gene ID: 221120

NCBI Accession: NP_631917

UniProt: Q96Q83

Pathways: DNA Damage Repair