E2F2 antibody (C-Term)

Catalog No. ABIN6748512

The Rabbit Polyclonal anti-E2F2 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect E2F2 in samples from Human, Mouse, Rabbit, Dog, Horse, Monkey and Bat.

Quick Overview for E2F2 antibody (C-Term) (ABIN6748512)

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Reactivity: Human, Mouse, Rabbit, Dog, Horse, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-E2F2 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human E2F2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Rabbit, Horse (100%) Bovine (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human E2F2 (Q14209, NP_004082). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Panda, Dog, Bat, Rabbit, Horse (100%), Elephant, Bovine (91%), Rat, Hamster, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (90%), Platypus (83%), Xenopus (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Alternative Name: E2F2

Background: Name/Gene ID: E2F2

Synonyms: E2F2, E2F-2, E2F transcription factor 2, Transcription factor E2F2

Gene ID: 1870

NCBI Accession: NP_004082

UniProt: Q14209

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication