PON1 antibody (AA 215-264)

Catalog No. ABIN6748518

The Rabbit Polyclonal anti-PON1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect PON1 in samples from Human, Rabbit and Pig.

Quick Overview for PON1 antibody (AA 215-264) (ABIN6748518)

Target: PON1 (Paraoxonase 1 (PON1))

Reactivity: Human, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PON1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PON1 Antibody

Binding Specificity: AA 215-264

Brand: IHC-plus™

Specificity: Human PON1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rabbit, Pig (100%) Mouse, Rat, Dog (92%) Bovine (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa215-264 of human PON1 (P27169, NP_000437). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Galago, Rabbit, Pig (100%), Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Horse (92%), Bovine (91%), Elephant (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PON1

Target: PON1 (Paraoxonase 1 (PON1))

Alternative Name: PON1 / ESA

Background: Name/Gene ID: PON1

Synonyms: PON1, A-esterase 1, Aromatic esterase 1, Esterase A, ESA, K-45, MVCD5, Paraoxonase 1, PON 1, Serum aryldialkylphosphatase 1, Arylesterase B-type, Paraoxonase B-type, PON, Serum aryldiakylphosphatase

Gene ID: 5444

NCBI Accession: NP_000437

UniProt: P27169