Klotho antibody (AA 251-300)

Catalog No. ABIN958310

The Rabbit Polyclonal anti-Klotho antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Klotho in samples from Mouse, Human, Rat, Rabbit, Horse, Dog, Guinea Pig, Bat and Monkey.

Quick Overview for Klotho antibody (AA 251-300) (ABIN958310)

Target: Klotho (KL)

Reactivity: Mouse, Human, Rat, Rabbit, Horse, Dog, Guinea Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Klotho antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Klotho Antibody

Binding Specificity: AA 251-300

Brand: IHC-plus™

Specificity: Human KL / Klotho

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Guinea pig (100%) Bovine, Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of human KL (Q9UEF7-2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Rabbit, Horse, Guinea pig, Platypus (100%), Gibbon, Bovine, Pig, Opossum (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Klotho

Target: Klotho (KL)

Alternative Name: KL / Klotho

Background: Name/Gene ID: KL

Synonyms: KL, Klotho

Gene ID: 9365

UniProt: Q9UEF7

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Activity, Growth Factor Binding