Hexokinase 2 antibody (AA 74-123)

Catalog No. ABIN462257

The Rabbit Polyclonal anti-Hexokinase 2 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Hexokinase 2 in samples from Human, Mouse, Rat, Dog, Rabbit, Pig, Cow, Horse, Monkey, Guinea Pig, Zebrafish (Danio rerio), Hamster and Bat.

Quick Overview for Hexokinase 2 antibody (AA 74-123) (ABIN462257)

Target: Hexokinase 2 (HK2)

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Pig, Cow, Horse, Monkey, Guinea Pig, Zebrafish (Danio rerio), Hamster, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Hexokinase 2 Antibody

Binding Specificity: AA 74-123

Brand: IHC-plus™

Specificity: Human HK2 / Hexokinase 2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Chicken, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa74-123 of human HK2 (P52789, NP_000180). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Sablefish, Stickleback (92%), Orangutan, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Zebrafish, Sea squirt (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Hexokinase 2

Target: Hexokinase 2 (HK2)

Alternative Name: HK2 / Hexokinase 2

Background: Name/Gene ID: HK2
Subfamily: Hexokinase
Family: Non-protein Kinase

Synonyms: HK2, Hexokinase II, Hexokinase type II, Hexokinase-2, muscle, HK II, HXK2, Hexokinase-2, Muscle form hexokinase, Hexokinase 2, HKII, Hxk 2

Gene ID: 3099

NCBI Accession: NP_000180

UniProt: P52789

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect