UCP2 antibody (AA 201-309)

Catalog No. ABIN675428

There are 2+ publications for this product available. The Rabbit Polyclonal anti-UCP2 antibody is suitable to detect UCP2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF (cc), IF (p), IHC (p), FACS and IHC (fro).

Quick Overview for UCP2 antibody (AA 201-309) (ABIN675428)

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-UCP2 Antibody

Binding Specificity: AA 201-309

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Mouse UCP-2 (Mitochondrial intermembrane space domain)

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-UCP2 antibody (ABIN675428)

Bo, Xie, Guo, Zhang, Guan, Li, Lu, Meng: "Methylglyoxal Impairs Insulin Secretion of Pancreatic β-Cells through Increased Production of ROS and Mitochondrial Dysfunction Mediated by Upregulation of UCP2 and MAPKs." in: Journal of diabetes research, Vol. 2016, pp. 2029854, (2016) (PubMed).

Chuang, Lin, Huang, Chang, Liou, Chen, Chang, Chan et al.: "Peroxisome proliferator-activated receptors ?/mitochondrial uncoupling protein 2 signaling protects against seizure-induced neuronal cell death in the hippocampus following experimental status ..." in: Journal of neuroinflammation, Vol. 9, pp. 184, (2012) (PubMed).

Target Details for UCP2

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Alternative Name: UCP-2

Background:

Synonyms: Slc25a8, Mitochondrial uncoupling protein 2, UCP 2, Solute carrier family 25 member 8, UCPH, Ucp2

Background: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat (By similarity).

Gene ID: 22228

UniProt: P70406

Pathways: Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Proton Transport