PPP3CA antibody (AA 432-481)

Catalog No. ABIN6755321

Product Details anti-PPP3CA Antibody

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Binding Specificity: AA 432-481

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Pig, Chicken, Xenopus laevis, Monkey, Guinea Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP3CA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PPP3CA / Calcineurin A

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa432-481 of human PPP3CA (Q08209, NP_000935). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback (100%), Zebrafish (90%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PPP3CA

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Alternative Name: PPP3CA / CCN1 / Calcineurin A (PPP3CA Products)

Background: Name/Gene ID: PPP3CA
Subfamily: PP2B
Family: Protein Phosphatase

Synonyms: PPP3CA, Calcineurin A, CALN, CALNA, CALNA1, CNA1, PPP2B, Calcineurin A alpha, CAM-PRP catalytic subunit, CCN1, CNA, CNA alpha, pp2b-alpha

Gene ID: 5530

NCBI Accession: NP_000935

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Synaptic Membrane, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling