PPP3CA antibody

Catalog No. ABIN6940381

The Mouse Monoclonal anti-PPP3CA antibody (Clone CALNA-2353) (ABIN6940381) specifically detects PPP3CA in IHC. The antibody is reactive with Human samples.

Quick Overview for PPP3CA antibody (ABIN6940381)

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPP3CA antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: CALNA-2353

Product Details anti-PPP3CA Antibody

Purpose: Calcineurin A / PPP3CA Antibody

Specificity: Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59 kDa catalytic A subunit and a 19 kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Calcineurin A protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: HeLa, MCF-7 or A431 cells. Prostate, Thyroid or Pancreas.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PPP3CA

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Alternative Name: Calcineurin A / PPP3CA

Background:

Calcineurin A alpha, Calcineurin A1, Calcineurin A, Calmodulin-dependent calcineurin A subunit alpha isoform, CALN, CALNA1, CAM-PRP catalytic subunit, CCN1, CNA alpha, CNA1, Ppp3ca, Protein phosphatase 2B catalytic subunit (PPP2B), Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform (PPP3CA)

HeLa, MCF-7 or A431 cells. Prostate, Thyroid or Pancreas.

Molecular Weight: 62kDa

Gene ID: 5530, 435512

UniProt: Q08209

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Synaptic Membrane, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling