Retinoblastoma Binding Protein 4 antibody (AA 71-120)

Catalog No. ABIN6755508

This Rabbit Polyclonal antibody specifically detects Retinoblastoma Binding Protein 4 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Dog, Chicken, Xenopus laevis, Monkey, Pig and Bat.

Quick Overview for Retinoblastoma Binding Protein 4 antibody (AA 71-120) (ABIN6755508)

Target: Retinoblastoma Binding Protein 4 (RBBP4)

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Dog, Chicken, Xenopus laevis, Monkey, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma Binding Protein 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Retinoblastoma Binding Protein 4 Antibody

Binding Specificity: AA 71-120

Specificity: Human RBBP4 / RBAP48

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human RBBP4 (Q09028, NP_005601). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Salmon, Stickleback, Zebrafish (100%), Pike (93%), Sea squirt, Drosophila, Beetle (92%), Pufferfish (91%), Galago (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Retinoblastoma Binding Protein 4

Target: Retinoblastoma Binding Protein 4 (RBBP4)

Alternative Name: RBBP4 / RBAP48

Background: Name/Gene ID: RBBP4

Synonyms: RBBP4, CAF-I p48, CAF-1 subunit C, CAF-I 48 kDa subunit, MSI1 protein homolog, Histone-binding protein RBBP4, NURF55, RBBP-4, RBAP48

Gene ID: 5928

NCBI Accession: NP_005601

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Stem Cell Maintenance, Chromatin Binding, Protein targeting to Nucleus