MEK1 antibody (AA 1-12)

Catalog No. ABIN6757368

This anti-MEK1 antibody is a Rabbit Polyclonal antibody detecting MEK1 in WB and ELISA. Suitable for Human, Mouse, Rat, Dog, Monkey, Cow, Rabbit, Pig, Xenopus laevis and Hamster.

Quick Overview for MEK1 antibody (AA 1-12) (ABIN6757368)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human, Mouse, Rat, Dog, Monkey, Cow, Rabbit, Pig, Xenopus laevis, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MEK1 Antibody

Binding Specificity: AA 1-12

Specificity: Detects an ~48kD protein, corresponding to the apparent molecular mass of Mek1 in rat, human, mouse, and Xenopus. This ~48kD band can be specifically inhibited by the relevant peptide. Species sequence Homology: Hamster and rabbit.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Pig, Xenopus (100%) Opossum, Chicken (92%) Platypus (83%).

Purification: Protein A purified

Immunogen: A 12 residue synthetic peptide MPKKKPTPIQLN(C) based on the rat Mek1 (residues 1-12) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Pig, Xenopus (100%), Opossum, Chicken (92%), Platypus (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB

Usage: Suitable for use in ELISA and Western Blot. Western Blot (Colorimetric): 1 μg/mL. Positive control: H1838-01 HeLa Cell Lysate, Heat Shocked.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1 / MEK1

Background: Name/Gene ID: MAP2K1
Subfamily: MAP2K
Family: Protein Kinase

Synonyms: MAP2K1, ERK activator kinase 1, MAPK/ERK kinase 1, MAPKK 1, MKK1, PRKMK1, MEK 1, MAP kinase kinase 1, MAPKK1, MEK1

Gene ID: 5604

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling