IGF1R antibody (pTyr1316)

Catalog No. ABIN6757514

The Mouse Monoclonal anti-IGF1R antibody has been validated for WB and ELISA. It is suitable to detect IGF1R in samples from Human and Mouse.

Quick Overview for IGF1R antibody (pTyr1316) (ABIN6757514)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGF1R antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IGF1R Antibody

Binding Specificity: pTyr1316

Specificity: Recognizes human IGF1 Receptor phosphorylated at Tyr1316. Species cross-reactivity: Mouse.

Purification: Gel filtration

Immunogen: Synthetic peptide corresponding to IGF1R (insulin-like growth factor 1 receptor) phosphorylated at Tyr1316.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.1 μg/mL), WB (0.5 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. ELISA: 0.1 μg/mL. Western Blot: 0.5 μg/mL for HRPO/ECL detection. Note: Recommended blocking buffer CPPT: 0.5 % casein, 1 % PEG 4000, 1 % polyvinypyrrolidone (PVP), 0.1 % Tween 20, 10 mM Tris/HCL, ph 7.4, 150 mM NaC. Positive control: I7661-18M1: Insulin-Like Growth Factor I Receptor, phosphorylated (Tyr1316) Positive Control (IGF1R). Supplied as a liquid cell lysate of 2x1E6 293 cells treated with insulin. Add 100 μL SDS-PAGE sample buffer and incubate at 90?C for 5 minutes. Aliquot and store at -20?C.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide, PEG, sucrose, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: IGF1R / IGF1 Receptor

Background: Name/Gene ID: IGF1R
Subfamily: Insulin receptor
Family: Protein Kinase

Synonyms: IGF1R, CD221 antigen, CD221, IGF1 Receptor, IGFR, IGF-I receptor, Soluble IGF1R variant 1, Soluble IGF1R variant 2, Type 1 IGF receptor, IGFIR, JTK13

Gene ID: 3480

UniProt: P08069

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy