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Tyrosine Hydroxylase antibody (C-Term)

This anti-Tyrosine Hydroxylase antibody is a Mouse Monoclonal antibody detecting Tyrosine Hydroxylase in WB, IHC and IHC (fro). Suitable for Human, Rat, Mouse and Monkey.
Catalog No. ABIN6757539

Quick Overview for Tyrosine Hydroxylase antibody (C-Term) (ABIN6757539)

Target

See all Tyrosine Hydroxylase (TH) Antibodies
Tyrosine Hydroxylase (TH)

Reactivity

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Human, Rat, Mouse, Monkey

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Tyrosine Hydroxylase antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Binding Specificity

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    C-Term

    Specificity

    Recognizes an epitope within the catalytically-active C-terminal end-product. This C-terminal product is ~34kD. (This end product is the result of a partial digestion of the rat TH with chymotrypsin.) Species cross-reactivity: Human, primate, mouse.

    Purification

    Immunoaffinity purified

    Immunogen

    Rat tyrosine hydroxylase.

    Isotype

    IgM
  • Application Notes

    Approved: IHC, IHC-Fr, WB (1:1000 - 1:2000)

    Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000-1:2000. Immunohistochemistry: 1:1000 -1:2000 in PBS/0.3 % Triton X-100 Cy3 Technique 1:4000-1:8000 in PBS/0.3 % Triton X-100 Bn-Av/HRP Technique. Control Tissue: Rat striatum and substantia nigra. Perfusion Fixation: Fixative-4 % paraformaldehyde in 0.1 M Phosphate buffer,  pH 7.4. Post Fixation-1.5 hr. at 4? C in 4 % paraformaldehyde in 0.1 M Phosphate buffer,  pH 7.4. Sections: 10um cryostat frozen sections or 50um vibratome sections.

    Comment

    Target Species of Antibody: Rat

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    deionized water

    Concentration

    Lot specific

    Buffer

    Lyophilized.
  • Target

    Tyrosine Hydroxylase (TH)

    Alternative Name

    TH / Tyrosine Hydroxylase

    Background

    Name/Gene ID: TH
    Family: Monooxygenase

    Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase

    Gene ID

    7054

    UniProt

    P07101

    Pathways

    Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
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