Prosaposin antibody (AA 421-524)

Catalog No. ABIN678923

The Rabbit Polyclonal anti-Prosaposin antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect Prosaposin in samples from Human.

Quick Overview for Prosaposin antibody (AA 421-524) (ABIN678923)

Target: Prosaposin (PSAP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prosaposin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Prosaposin Antibody

Binding Specificity: AA 421-524

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PSAP

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Prosaposin

Target: Prosaposin (PSAP)

Alternative Name: PSAP

Background:

Synonyms: A1 activator, Cerebroside sulfate activator, Co-beta-glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, Proactivator polypeptide precursor, Prosaposin sphingolipid activator protein 1, prosaposin variant Gaucher disease and variant metachromatic leukodystrophy, Protein A, Protein C, PSAP, SAP-1, SAP-2, SAP_HUMAN, SAP1, Saposin A, Saposin B, Saposin B Val, Saposin C, Saposin-D, Saposins, Sphingolipid activator protein 1, Sphingolipid activator protein 2, Sulfated glycoprotein 1, Sulfatide/GM1 activator.

Background: PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D, which are similar to each other and are sphingolipid hydrolase activator proteins. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in PSAP gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy.

Gene ID: 5660

UniProt: P07602

Pathways: Positive Regulation of Endopeptidase Activity