NR1I2 antibody (AA 51-150)

Catalog No. ABIN679598

The Rabbit Polyclonal anti-NR1I2 antibody has been validated for WB, ELISA, FACS and IHC (p). It is suitable to detect NR1I2 in samples from Human, Mouse and Pig. There are 2+ publications available.

Quick Overview for NR1I2 antibody (AA 51-150) (ABIN679598)

Target: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Reactivity: Human, Mouse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR1I2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NR1I2 Antibody

Binding Specificity: AA 51-150

Cross-Reactivity: Human, Mouse, Pig

Predicted Reactivity: Rat,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PXR

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NR1I2 antibody (ABIN679598)

Zhou, Li, Wang, Jin, Shi, Wang, Bi: "Cecropin B Represses CYP3A29 Expression through Activation of the TLR2/4-NF-κB/PXR Signaling Pathway." in: Scientific reports, Vol. 6, pp. 27876, (2016) (PubMed).

Sehirli, Cetinel, Ozkan, Selman, Tetik, Yuksel, Dulger: "St. John's wort may ameliorate 2,4,6-trinitrobenzenesulfonic acid colitis off rats through the induction of pregnane X receptors and/or P-glycoproteins." in: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, Vol. 66, Issue 2, pp. 203-14, (2015) (PubMed).

Target Details for NR1I2

Target: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Alternative Name: PXR

Background:

Synonyms: BXR, PAR, PRR, PXR, SAR, SXR, ONR1, PAR1, PAR2, PARq, Nuclear receptor subfamily 1 group I member 2, Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, Pregnane X receptor, Steroid and xenobiotic receptor, NR1I2

Background: Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.

Gene ID: 8856

UniProt: O75469

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway