TNF alpha antibody (AA 80-235) (Biotin)

Catalog No. ABIN2629353

The Rabbit Polyclonal anti-TNF alpha antibody has been validated for WB. It is suitable to detect TNF alpha in samples from Rat.

Quick Overview for TNF alpha antibody (AA 80-235) (Biotin) (ABIN2629353)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is conjugated to Biotin

Application: Western Blotting (WB)

Product Details anti-TNF alpha Antibody (Biotin)

Binding Specificity: AA 80-235

Specificity: Rat TNF Alpha

Purification: Affinity purified

Immunogen: Recombinant TNFa (Leu80-Leu235) expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: WB (1:100 - 1:400)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.02 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Store frozen product at or below -20°C. Thawed product may be stored for 2-4 weeks at 4°C. For optimal storage, aliquot to smaller portions and store at -20°C to -70°C. Avoid freeze-thaw cycles.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome