SMO antibody (AA 551-650)

Catalog No. ABIN680908

This Rabbit Polyclonal antibody specifically detects SMO in WB, ELISA, IHC (p) and IF (p). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for SMO antibody (AA 551-650) (ABIN680908)

Target: SMO (Smoothened Homolog (Drosophila) (SMO))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-SMO Antibody

Binding Specificity: AA 551-650

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Smoothened

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SMO antibody (ABIN680908)

Zhou, Zhou, Liu, Shen: "GDC-0449 improves the antitumor activity of nano-doxorubicin in pancreatic cancer in a fibroblast-enriched microenvironment." in: Scientific reports, Vol. 7, Issue 1, pp. 13379, (2019) (PubMed).

Wang, Zhang, Bai, Wang, Du: "Baicalin Attenuates Alcoholic Liver Injury through Modulation of Hepatic Oxidative Stress, Inflammation and Sonic Hedgehog Pathway in Rats." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 39, Issue 3, pp. 1129-40, (2016) (PubMed).

Target Details for SMO

Target: SMO (Smoothened Homolog (Drosophila) (SMO))

Alternative Name: Smoothened homolog

Background:

Synonyms: Gx, SMOH, FZD11, Smoothened homolog, SMO, Protein Gx

Background: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.

Gene ID: 6608

UniProt: Q99835

Pathways: Hedgehog Signaling