HES1 antibody (AA 41-150)

Catalog No. ABIN682393

This Rabbit Polyclonal antibody specifically detects HES1 in WB, ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for HES1 antibody (AA 41-150) (ABIN682393)

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HES1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HES1 Antibody

Binding Specificity: AA 41-150

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HES1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-HES1 antibody (ABIN682393)

Shi, Shu, Yang, Xu, Xing, Liu, Chen, Qi, Liu, Wang, Tang, Xie: "Wnt and Notch signaling pathway involved in wound healing by targeting c-Myc and Hes1 separately." in: Stem cell research & therapy, Vol. 6, pp. 120, (2015) (PubMed).

Gao, Zhang, Wang, Liu, Zheng, Yang, Huang, Ye, Luo, Xiao: "Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer." in: Scientific reports, Vol. 4, pp. 3963, (2014) (PubMed).

Long, Qiu, Liu, Fei, Feng, Wang, Zhong, Yi, Liu, Zhang, Han: "Functional recovery and neuronal regeneration of a rat model of epilepsy by transplantation of Hes1-down regulated bone marrow stromal cells." in: Neuroscience, Vol. 212, pp. 214-24, (2012) (PubMed).

Target Details for HES1

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Alternative Name: HES1

Background:

Synonyms: HHL, HRY, HES-1, bHLHb39, Transcription factor HES-1, Class B basic helix-loop-helix protein 39, Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein, HES1, HL

Background: Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

Gene ID: 3280

UniProt: Q14469

Pathways: DNA Damage Repair