TP53BP1 antibody (pSer25, pSer29)

Catalog No. ABIN682993

This Rabbit Polyclonal antibody specifically detects TP53BP1 in ELISA, ICC, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for TP53BP1 antibody (pSer25, pSer29) (ABIN682993)

Target: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TP53BP1 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TP53BP1 Antibody

Binding Specificity: pSer25, pSer29

Specificity: This phosphorylated site is the same in Mouse and homologous to p53BP1 Ser30 + Ser34 in Rat.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human p53BP1 around the phosphorylation site of Ser25/29

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-TP53BP1 antibody (ABIN682993)

Cai, Cao, Zhang, Xue, Zhang, Zhou, Zhou, Sun, Fu: "Oncogenic miR-17/20a Forms a Positive Feed-forward Loop with the p53 Kinase DAPK3 to Promote Tumorigenesis." in: The Journal of biological chemistry, Vol. 290, Issue 32, pp. 19967-75, (2015) (PubMed).

Target Details for TP53BP1

Target: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Alternative Name: p53BP1 +

Background:

Synonyms: p202, 53BP1, Tumor suppressor p53-binding protein 1, p53-binding protein 1, p53BP1, TP53BP1

Background: Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation.

Gene ID: 7158

UniProt: Q12888

Pathways: DNA Damage Repair