Cyclin B1 antibody (pSer147)

Catalog No. ABIN684448

Product Details anti-Cyclin B1 Antibody

Target: Cyclin B1 (CCNB1)

Binding Specificity: pSer147

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser147

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Cyclin B1 antibody (ABIN684448)

Hawash, Kahraman, Eren, Cetin Atalay, Baytas: "Synthesis and biological evaluation of novel pyrazolic chalcone derivatives as novel hepatocellular carcinoma therapeutics." in: European journal of medicinal chemistry, Vol. 129, pp. 12-26, (2017) (PubMed).

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1 (CCNB1 Products)

Synonyms: ccnb antibody, cycb antibody, cb267 antibody, cycb1 antibody, wu:fa19g04 antibody, wu:fb16d01 antibody, wu:fb16e07 antibody, wu:fi21c01 antibody, ccnb1 antibody, MGC53596 antibody, CCNB antibody, Ccnb1-rs1 antibody, Ccnb1-rs13 antibody, CycB1 antibody, Cycb-4 antibody, Cycb-5 antibody, Cycb1-rs1 antibody, cyclin B1 antibody, cyclin B1 S homeolog antibody, ccnb1 antibody, ccnb1.2 antibody, ccnb1.S antibody, CCNB1 antibody, Ccnb1 antibody, ccnb1.2.S antibody

Background:

Synonyms: Cyclin B1 phospho S147, Cyclin B1 Ser147, p-Cyclin B1 S147, p-Cyclin B1 Ser147, p-Cyclin B1 phospho S147, CCNB 1, CCNB, CCNB1, CCNB1_HUMAN, G2 mitotic specic cyclin B1, G2/mitotic-specic cyclin-B1.

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].

Gene ID: 891

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase