Cyclin E1 antibody (pThr77) (HRP)

Catalog No. ABIN684502

This Rabbit Polyclonal antibody specifically detects Cyclin E1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Cyclin E1 antibody (pThr77) (HRP) (ABIN684502)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-Cyclin E1 Antibody (HRP)

Binding Specificity: pThr77

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Cyclin E around the phosphorylation site of Thr77

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E

Background:

Synonyms: CCNE 1, CCNE,CCNE1,Cyclin E1 antibody Cyclin Es,Cyclin Et,CyclinE, G1/S specic cyclin E, CCNE1_HUMAN.

Background: Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Molecular Weight: 47kDa

Gene ID: 898

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases