GADD45A antibody (AA 65-165) (Cy5)

Catalog No. ABIN685436

The Rabbit Polyclonal anti-GADD45A antibody has been validated for WB, FACS, IF (cc) and IF (p). It is suitable to detect GADD45A in samples from Human, Rat and Mouse.

Quick Overview for GADD45A antibody (AA 65-165) (Cy5) (ABIN685436)

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GADD45A antibody is conjugated to Cy5

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-GADD45A Antibody (Cy5)

Binding Specificity: AA 65-165

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GADD45

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GADD45A

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Alternative Name: GADD45

Background:

Synonyms: DDIT1, GADD45, Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein, DDIT-1, GADD45A

Background: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes, stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.

Gene ID: 1647

UniProt: P24522

Pathways: p53 Signaling, Cell Division Cycle