GPER antibody (AA 251-375)

Catalog No. ABIN685717

This Rabbit Polyclonal antibody specifically detects GPER in WB, FACS, IF (cc), IF (p), IHC (p), ELISA and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for GPER antibody (AA 251-375) (ABIN685717)

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPER antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GPER Antibody

Binding Specificity: AA 251-375

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GPR30

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-GPER antibody (ABIN685717)

Wang, Alencar, Lin, Sun, Sudo, Zapata-Sudo, Lowe, Groban: "Activation of GPR30 improves exercise capacity and skeletal muscle strength in senescent female Fischer344 × Brown Norway rats." in: Biochemical and biophysical research communications, Vol. 475, Issue 1, pp. 81-6, (2016) (PubMed).

Wang, Zhao, Lin, Groban: "Activation of GPR30 inhibits cardiac fibroblast proliferation." in: Molecular and cellular biochemistry, Vol. 405, Issue 1-2, pp. 135-48, (2015) (PubMed).

Zhao, Wang, Lin, Groban: "GPR30 decreases cardiac chymase/angiotensin II by inhibiting local mast cell number." in: Biochemical and biophysical research communications, Vol. 459, Issue 1, pp. 131-6, (2015) (PubMed).

Target Details for GPER

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Alternative Name: GPR30

Background:

Synonyms: mER, CEPR, GPER, DRY12, FEG-1, GPR30, LERGU, LyGPR, CMKRL2, LERGU2, GPCR-Br, G-protein coupled estrogen receptor 1, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1, G protein-coupled estrogen receptor 1, G-protein coupled receptor 30, IL8-related receptor DRY12, Lymphocyte-derived G-protein coupled receptor, Membrane estrogen receptor, GPER1

Background: G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK. Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems. Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner. Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation. Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells. Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation. Stimulates uterine epithelial cell proliferation. Enhances uterine contractility in response to oxytocin. Contributes to thymic atrophy by inducing apoptosis. Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules. Promotes neuritogenesis in developing hippocampal neurons. Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death. Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons. Inhibits early osteoblast proliferation at growth plate during skeletal development. Inhibits mature adipocyte differentiation and lipid accumulation. Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells.

Gene ID: 2852

UniProt: Q99527

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process