ARFIP2 antibody (AA 221-341) (Cy5)

Catalog No. ABIN686591

The Rabbit Polyclonal anti-ARFIP2 antibody is suitable to detect ARFIP2 in samples from Human. It has been validated for WB, IF (cc) and IF (p).

Quick Overview for ARFIP2 antibody (AA 221-341) (Cy5) (ABIN686591)

Target: ARFIP2 (ADP-Ribosylation Factor Interacting Protein 2 (ARFIP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARFIP2 antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ARFIP2 Antibody (Cy5)

Binding Specificity: AA 221-341

Purpose: Arfaptin 2 Polyclonal Antibody, Cy5 Conjugated

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Arfaptin 2

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ARFIP2

Target: ARFIP2 (ADP-Ribosylation Factor Interacting Protein 2 (ARFIP2))

Alternative Name: Arfaptin 2

Background:

Synonyms: ARFIP2, arfaptin 2, ADP-ribosylation factor interacting protein 2, Partner of RAC1, POR1, ARFP2_HUMAN .

Background: ARFIP2 is a ubiquitously expressed protein implicated in mediating cross talk between RAC and ARF small GTPases. It has been shown that ARFIP2 binds specifically to GTP-bound ARF1 and ARF6, but binds to Rac-GTP and Rac-GDP with similar affinities. The X-ray structure of arfaptin reveals an elongated, crescent-shaped dimer of 3-helix coiled-coils. Structures of arfaptin with Rac bound to either GDP or the slowly hydrolysable analog GMPPNP show that the switch regions adopt similar conformations in both complexes.

Gene ID: 23647