K-RAS antibody
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- Target See all K-RAS (KRAS) Antibodies
- K-RAS (KRAS) (GTPase Kras (KRAS))
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Reactivity
- Human, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This K-RAS antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human, Rat
- Predicted Reactivity
- Mouse,Dog,Cow,Sheep,Pig,Horse
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human Pan-ras
- Isotype
- IgG
- Top Product
- Discover our top product KRAS Primary Antibody
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anti-GTPase Kras (KRAS) (AA 1-188) antibody
KRAS Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 3B10-2F2 unconjugated
anti-GTPase Kras (KRAS) (AA 16-125) antibodyKRAS Reactivity: Human WB, ELISA Host: Mouse Monoclonal 4F3 unconjugated
anti-GTPase Kras (KRAS) (AA 146-174) antibodyKRAS Reactivity: Human, Rat, Mouse WB, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated
anti-GTPase Kras (KRAS) (AA 2-168) antibodyKRAS Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-GTPase Kras (KRAS) (AA 25-130) antibodyKRAS Reactivity: Human, Rat, Mouse WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-GTPase Kras (KRAS) (N-Term) antibodyKRAS Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Cow, Drosophila melanogaster, Pig, Bat, Chicken, Xenopus laevis, Hamster, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-GTPase Kras (KRAS) (pSer89) antibodyKRAS Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-GTPase Kras (KRAS) (N-Term) antibodyKRAS Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Cow, Saccharomyces cerevisiae WB Host: Rabbit Polyclonal unconjugated
anti-GTPase Kras (KRAS) (AA 1-189) antibodyKRAS Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-GTPase Kras (KRAS) (C-Term) antibodyKRAS Reactivity: Human, Rat, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- K-RAS (KRAS) (GTPase Kras (KRAS))
- Alternative Name
- GTPase Kras (KRAS Products)
- Background
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Synonyms: K-Ras, P21 protein, p21ras, H-Ras-1, c-H-ras, N-Ras.
Background: RAS superfamily comprises around 50 related genes encoding GTP-binding domain (G-proteins) involved in signal transduction. The main genes are HRAS, NRAS and KRAS. Ras proteins are membrane-bound GTPases. The inactive form is GDP-bound. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, colony-stimulating factor and fibroblast growth factor. These kinases transiently convert RAS-GDP to RAS-GTP, the active form of RAS. Single amino acid substitutions can activate RAS making it highly oncogenic. Such mutations generally reduce the GTPase activity of RAS, prolonging it in its active GTP-bound form. The consequence of this is sustained activation of the RAF1-MAPK signalling pathway. RAS mutations are found in 10-15 % of tumours. A high incidence of RAS mutations is found in pancreatic cancers.
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