Cyclin D1 antibody (PE)

Catalog No. ABIN6934200

The Mouse Monoclonal anti-Cyclin D1 antibody is suitable to detect Cyclin D1 in samples from Human, Mouse, Rat and Monkey. It has been validated for FACS, IF and T.

Quick Overview for Cyclin D1 antibody (PE) (ABIN6934200)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin D1 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Clone: DCS-6

Product Details anti-Cyclin D1 Antibody (PE)

Specificity: Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Purification: Purified by Protein A/G

Immunogen: Human recombinant full length cyclin D1 protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. ~60 % of mantle cell lymphomas and ~40 % of breast carcinomas are positive.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100 for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1

Molecular Weight: 36kDa

Gene ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling