Glypican 3 antibody (AA 511-580) (CF®555)

Catalog No. ABIN6934330

This anti-Glypican 3 antibody is a Mouse Monoclonal antibody detecting Glypican 3 in IF, FACS, IHC and StM. Suitable for Human.

Quick Overview for Glypican 3 antibody (AA 511-580) (CF®555) (ABIN6934330)

Target: Glypican 3 (GPC3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glypican 3 antibody is conjugated to CF®555

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: SPM595

Product Details anti-Glypican 3 Antibody (CF®555)

Binding Specificity: AA 511-580

Purification: Purified by Protein A/G

Immunogen: A recombinant fragment containing amino acids 511-580 of human glypican-3

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: 293T cells. Hepatocellular carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: GPC3

Background: Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100 % in follicular carcinoma and 70 % in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.

Molecular Weight: 67kDa

Gene ID: 2719

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process