ARF1 antibody (AA 174-180)

Catalog No. ABIN6936427

This anti-ARF1 antibody is a Mouse Monoclonal antibody detecting ARF1 in WB, ELISA, IF, Coat and IHC (f). Suitable for Human.

Quick Overview for ARF1 antibody (AA 174-180) (ABIN6936427)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 3F1

Product Details anti-ARF1 Antibody

Binding Specificity: AA 174-180

Specificity: The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide SNQLRNQ, corresponding to C terminal amino acids 174-180 of Human ARF1.

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Kidney, Liver, Brain or Breast.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ARF1

Background: ADP-ribosylation factor 1, ARF1,ARF1 (Golgi Apparatus Marker)
Cellular localisation: Cytoplasmic (Golgi Apparatus)

Molecular Weight: 20kDa

Gene ID: 375, 286221

UniProt: P84077

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process