Recombinant Aurora Kinase B antibody (AA 89-251)

Catalog No. ABIN6936445

The Rabbit Monoclonal anti-Aurora Kinase B antibody (Clone AURKB-3121R) (ABIN6936445) specifically detects Aurora Kinase B in IHC and WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant Aurora Kinase B antibody (AA 89-251) (ABIN6936445)

Target: Aurora Kinase B (AURKB)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: AURKB-3121R

Product Details anti-Aurora Kinase B Antibody

Binding Specificity: AA 89-251

Purpose: Recombinant Aurora B (Proliferation Marker) Antibody

Specificity: Recognizes a protein of 39 kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Tonsil, Placenta, Hodgkin's Lymphoma or Colon Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: AURKB

Background:

AIK2; AIM-1; ARK-2; AurB; AURKB; Aurora-1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora-B; Aurora-related kinase 2; Aurora/IPL1-related kinase 2; IPL1; Protein phosphatase 1 regulatory subunit 48 (PPP1R48); Serine/threonine-protein kinase 12; Serine/threonine-protein kinase aurora-B; STK1; STK12; STK5

HeLa cells. Tonsil, Hodgkin's Lymphoma or Colon Carcinoma., Placenta, Human Liver

Molecular Weight: 39kDa

Gene ID: 9212, 442658

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades